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A Centralized Model for Creating Shared, Standardized, Microsatellite Data that Simplifies Inter-laboratory Collaboration

Report

Abstract

We demonstrate an efficient model for standardizing microsatellite DNA data among laboratories studying Oncorhynchus mykiss. Eight laboratories standardized 13 microsatellite loci following allele nomenclature of a central laboratory (average inter-laboratory genotyping concordance [98%]). Following this central model, we have currently standardized 298 alleles from throughout the species native range. Although we focus here on ,O. mykiss, our experiences and recommendation apply equally to other broadly distributed species that may benefit from multi-laboratory collaborative data collection.

Authors

Jeff Stephenson, Matt R Campbell, Jon E Hess, Chris Kozfkay, Andrew P Matala, Megan V McPhee, Paul Moran, Shawn R Narum, Melanie M Paquin, Ora Schlei, Maureen P Small, Donald M Van Doornik, and John K Wenburg

Citation

Stephenson J.J. et al. 2009. A Centralized Model for Creating Shared, Standardized, Microsatellite Data that Simplifies Inter-Laboratory Collaboration.  Conservation Genetics 10:1145-1149.

Date

Oct 22nd, 2008

Report No.

Stephenson_etal_CoGen_2009

Media Type

Journal Article

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