A Centralized Model for Creating Shared, Standardized, Microsatellite Data that Simplifies Inter-laboratory Collaboration
Request Report from the Columbia Basin Fish & Wildlife Library (formerly known as StreamNet Library)
We demonstrate an efficient model for standardizing microsatellite DNA data among laboratories studying Oncorhynchus mykiss. Eight laboratories standardized 13 microsatellite loci following allele nomenclature of a central laboratory (average inter-laboratory genotyping concordance [98%]). Following this central model, we have currently standardized 298 alleles from throughout the species native range. Although we focus here on ,O. mykiss, our experiences and recommendation apply equally to other broadly distributed species that may benefit from multi-laboratory collaborative data collection.
Jeff Stephenson, Matt R Campbell, Jon E Hess, Chris Kozfkay, Andrew P Matala, Megan V McPhee, Paul Moran, Shawn R Narum, Melanie M Paquin, Ora Schlei, Maureen P Small, Donald M Van Doornik, and John K Wenburg
Stephenson J.J. et al. 2009. A Centralized Model for Creating Shared, Standardized, Microsatellite Data that Simplifies Inter-Laboratory Collaboration. Conservation Genetics 10:1145-1149.
Oct 22nd, 2008